A three-vehicle collision that closed a portion of Highway in Scarborough 401 for hours on Sunday left two people seriously injured.

The crash happened on the eastbound express lanes of the highway near Kennedy Road just after 4:15 p.m.

Ontario Provincial Police said a transport truck rear-ended a passenger vehicle and then side-swiped another car.

401 collision

One person needed to be extricated following the collision, Toronto Fire said.

Two people were taken to trauma centre in serious but non-life-threatening condition, Toronto paramedics said, adding that five others sustained minor injuries.

The collision prompted a lengthy closure of all eastbound express lanes near Kennedy Road, leaving many stuck on the highway.

Highway 401 closure

Traffic cameras captured people getting out of their vehicles and standing on the road.

Some were also seen turning their vehicles around and driving in the opposite direction to get out of the traffic jam.

The highway reopened just after 8 p.m.